Title: Spectroscopic Profile as Pharmacognostic Criteria to Distinguish the Medicinally Important Plumbago Species

Abstract: The root of the Plumbago species were examined phytochemically and their potentials are well studied. The spectrometric and chromatographic screening methods could provide the needed preliminary observations to select crude plant extracts with potentially useful properties for further chemical and pharmacological investigations. Objective: The present study was aimed to reveal the inter-specific similarity and variation among the selected Plumbago species viz., Plumbago zeylanica Linn, Plumbago auriculata Lam, Plumbago rosea Linn. using UV-VIS and FTIR profiles.Methods: To know the various phytoconstituents and functional groups and similarity and variation present in the Plumbago species aerial parts, the UV-VIS spectroscopic analysis and FTIR analysis was carriedout using Shimazdu and Perkin Elmer spectrophotometer respectively. Result: The UV-VIS spectroscopic analysis revealed the presence of various functional compounds and groups and similarity and variation in the aerial parts of studied Plumbago species. The FTIR analysis identified various functional groups like alcohols, phenols, alkanes, alkynes, alkyl halides, aldehydes, carboxylic acids, aromatics, nitro compounds and amines presence in the crude powder of Plumbago species aerial parts. Conclusion: The results of the present study paved a way to predict and compare the phytoconstituents presence in all the three selected Plumbago species which can be used for further characterization. In addition it provides the pharmacognostical marker to distinguish the medicinally important Plumbago species using relatively simple, cost-effective spectroscopic profile.

Title: Heterosis for biochemical constituents and protein profiles of elite rice hybrids of Tamil Nadu

Abstract: Heterosis for grain yield is the outcome of several physiological as well as biochemical characters which have been relatively less investigated though their contribution is certain. Study was taken to assess the hybrid vigour of two high yielding rice hybrids namely CoRH 2(6.5t/ha) and ADTRH 1(7.0 t/ha) and their parents (IR 58025 A X C 20R; IR 58025 A X IR 66R respectively) in terms of some important physiological parameters such as chlorophyll content, soluble protein, variation in protein banding and nitrate reductase activity. All the physiological parameters were high in the hybrids than their parents throughout the crop growth period. Among the hybrids ADTRH 1, recorded higher chlorophyll content, soluble protein and Nitrate Reductase activity. The study on protein profiling showed differences in protein banding new protein bands between the parents and hybrids, while new bands of 90 kDa, 60kDa, 50kDa and 45kDa were present in the hybrids. Finally, the study revealed that ADTRH 1 performed better than CoRH 2.

Title: Comparative responses of four tree legumes plants to PEG-induced water stress at seedling stage

Abstract: Drought resistance is generally defined as the maintenance of plant production during moderate water deficit. In the present investigation, four tree species ( Bauhinia variegate Linn., Cassia fistula Linn., Delonix regia (Hook.) Raf. and Peltophorum pterocarpum (DC.) K. Heyne) of the family Leguminosae (Fabaceae) were characterized in respect of their responses to water stress. Seedlings of those species (20 days old) exposed to water stress (two levels) were analyzed for relative water content, chlorophyll, protein, carbohydrate and proline content. Considering the comparative biochemical analysis (i.e., loss of chlorophyll and protein, accumulation of sugars and proline) in leaves of seedlings of four investigated plant species, B. variegata and C. fistula , these two plants, showed higher potential for drought tolerance.